![]() Ten fold serial dilutions of the soil samples were made in distilled water and 0.1ml of 10 -5 to 10 -9 dilutions were plated on different media. Different media like Nutrient Agar, Lauria Bertani agar plates containing Lead and Mercury to a final concentration of 5.0ppm and 7.5ppm respectively. Soil samples were collected from the sites where lead and Mercury effluents were discharged. Screening of Lead and Mercury Degrading Bacteria The soil sample collected was dispensed in the sterile bags and sealed and the sample was brought to the laboratory. The soil sample was collected from a depth of 15 inches depth in to the soil where there was high moisture content, because the Bacteria need moisture content for growth. The soil samples containing the bacteria were collected from different areas of Tadaepalli and Mangalagiri, Guntur District of Andhra Pradesh where there was an accumulation of the petroleum spills for Lead deposits and where as for Mercury degradation samples were collected from Seashore of Suryalanka, Baptla, Guntur District, and Andhra Pradesh. In the present paper efforts have been made to isolate and identify the biodegrading bacteria from soil.Ĭollection of Soil Samples for Bacterial Strains Isolation This investment validates the need to research the utilization of microbial processes to clean up contaminated sites. It is anticipated that government as well as private industries will invest heavily to clean up polluted environments. Currently, more research is being performed on the use of microbes to degrade metals. Likewise, there are records that organisms like Escherichia and Pseudomonas are used in the biodegradation of copper (Barkay and Cowell,1983). ![]() For example Alcaligenes and Pseudomonas have been used in the biodegradation of chromium (Barkay and Colwell, 1983). These mechanisms include bioaugmentation, in which microbes and nutrients are added to the contaminated site, and biostimulation, in which nutrients and enzymes are added to supplement the intrinsic microbes of the site. ![]() Staphylococcus, Bacillus, Pseudomonas, Citrobacteia, Klebsilla, and Rhodococcus are organisms that are commonly used in biodegradation and bioremediation mechanisms. More recently, researchers have discovered that microbial processes can be used to cleanup radioactive and metallic compounds. For the last decade researchers have also discovered that biodegradation technology can be used at sites contaminated with solvents, explosives, polycyclic aromatic hydrocarbons (PAHs), and polychlorinated biphenyls (PCBs). Biodegradation was used over 100 years ago with the opening of the first biological sewage treatment plant in Sussex, UK, in 1891 however, the word “Biodegradation” is fairly new first appeared in a peer-reviewed scientific literature in 1987.īiodegradation can be a cost efficient and reliable method for removing hazardous waste from heavily contaminated sites with organic compounds. demonstrating that some form of biodegradation and bioremediation was practiced by humans since the beginning of recorded history. Evidence of kitchen middens (ancient household garbage dumps) and compost piles dates back to 6000 B.C. Available from: īiodegradation technology uses microorganisms to reduce, eliminate or transform the benign contaminants and their products which are present in soil, sediments, water and air. Isolation and Characterization of Bio-Degrading Bacteria from Soil Samples. KEYWORDS: Biodegradation Bacteria Soil Sampleĭownload this article as: Copy the following to cite this article:īabu T. The effect of various physico-chemical parameters on growth of these bacteria was also studied. Department of Biotechnology, P.G Courses and Research Centre, DNR College, Bhimvaram - 534 202 India.ĪBSTRACT: In the present study, a total of four species of biodegrading bacteria namely Bacillus subtilis, Eschericia coli, Staphylococcus aereus and Pseudomonas putida were isolated from soil samples collected from the sites where lead and mercury were discharged. ![]() Isolation and Characterization of Bio-Degrading Bacteria from Soil Samples
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |